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Nanosensor Technology at Draper
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Nanosensor Technology at Draper

Draper researchers are developing several types of nanosensors aimed at measuring ion and small molecule concentrations in the intracellular and extracellular environment. These nanosensors are composed of a variety of chemistries contained in a plasticized, fluorescent polymer bead. The beads are produced in-house in minutes, and can easily be modified to change the concentration range and detectable analytes. The optical nanosensor group focuses on two areas of interest: the production of new sensors to measure an ever increasing array of physiologically relevant analytes, and the application of these sensors to biological systems. Specific applications include sodium imaging in biological systems and in vivo glucose monitoring.

Glucose SchematicProposed clinical system for monitoring ion or glucose levels in patients

 

Technology Features:

Broad Range of Detection Targets
Optical nanosensors can be formulated to monitor a virtually limitless selection of ions (eg. sodium, potassium).

Small Size
Sensor size (~100nm diameter) allows both intra- and extracellular monitoring while reducing problems with rejection and fouling.

Advantageous Surface Chemistry
Sensors can be functionalized to ease intracellular loading, target physiological locations, promote desirable interactions, etc.

Tunable Sensitivity
Sensor range of detection can be tuned to physiologically relevant concentrations.

Biocompatible
Cytotoxicity tests confirm sensor biocompatibility in cell lines.

Long Life
First generation sensors have been confirmed to be stable for 1 week in solution and lifetime can be extended further; photobleaching is minimized through ratiometric measurements; second generation sensors eliminate photobleaching by incorporating quantum dots.

Real-time Response
Rapid sensor response facilitates real-time analysis of ion flux in localized regions intra- or extracellularly.

 

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